Bright field microscopy is the simplest of all the light microscopy techniques.
2.
For bright field microscopy, negative staining is typically performed using a black ink fluid such as nigrosin.
3.
The second flagellum is difficult to see with bright field microscopy, and was entirely overlooked by early observers.
4.
It is comparable to looking through a glass window : you ( bright field microscopy ) don't see the glass but merely the dirt on the glass.
5.
Oblique illumination suffers from the same limitations as bright field microscopy ( low contrast of many biological samples; low apparent resolution due to out of focus objects ).
6.
The various technologies differ in the collection process, possible imaging methods ( fluorescence microscopy / bright field microscopy / differential interference contrast microscopy / phase contrast microscopy / etc . ) and the types of holders and tissue preparation needed before the imaging and isolation.
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